Positron emission tomography with fluorodeoxyglucose-F18 in an animal model of mania

Hougland, Matthew Tyler; Gao, Yonglin; Herman, Laura; Ng, Chin K.; Lei, Zhenmin; El-Mallakh, Rif S.

doi:10.1016/j.pscychresns.2008.01.004

« Previous Next »Psychiatry Research: Neuroimaging
Volume 164, Issue 2 , Pages 166-171, 30 November 2008

Positron emission tomography with fluorodeoxyglucose-F18 in an animal model of mania

Matthew Tyler Hougland
- Department of Psychiatry and Behavioral Sciences, University of Louisville School of Medicine, Louisville, KY, United States
- Department of Psychology, University of Louisville School of Medicine, Louisville, KY, United States
Yonglin Gao
- Department of Psychiatry and Behavioral Sciences, University of Louisville School of Medicine, Louisville, KY, United States
Laura Herman
- Department of Psychiatry and Behavioral Sciences, University of Louisville School of Medicine, Louisville, KY, United States
Chin K. Ng
- Department of Psychology, University of Louisville School of Medicine, Louisville, KY, United States
Zhenmin Lei
- Department of Psychiatry and Behavioral Sciences, University of Louisville School of Medicine, Louisville, KY, United States
- Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Louisville, KY, United States
Rif S. El-Mallakh
- Department of Psychiatry and Behavioral Sciences, University of Louisville School of Medicine, Louisville, KY, United States
- Corresponding author. Mood Disorders Research Program, Department of Psychiatry, University of Louisville School of Medicine, MedCenter One, 501 E Broadway, Suite 340, Louisville, KY 40202, United States. Tel.: +1 502 852 1124; fax: +1 502 852 5098.

Received 28 April 2007; received in revised form 11 November 2007; accepted 7 January 2008.

Abstract

Intracerebroventricular (ICV) administration of ouabain to young adult rats has been suggested to model human bipolar mania. In the human condition, mania and bipolar depression are both associated with reductions in frontal cerebral metabolism. We utilized [¹⁸F]-fluorodeoxyglucose [¹⁸FDG] positron emission tomography (PET) to visualize glucose uptake in animals receiving ICV ouabain. Animals received 5 µl of 10⁻³ M ouabain ICV, were anesthetized with isoflurane inhalation, and administered intraperitoneally with 0.5 mCi of ¹⁸FDG. PET data were collected over 20 min 1 hour later. Additionally, the effect of lithium was examined in animals receiving lithium in their diet for 1 week before the ICV ouabain injection. Data were analyzed with IDL Virtual Machine software. Brain glucose utilization as measured by ¹⁸FDG uptake was significantly reduced in animals receiving ICV ouabain compared with those receiving equal volumes of artificial cerebrospinal fluid. Pretreatment with lithium normalized ¹⁸FDG uptake. These results mirror human studies.